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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and therefore may well contribute to tumorigenesis. This protein was located to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant part in advertising and marketing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two further splice variants ended up reported but in depth nucleotide sequence facts is not but readily available.
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Blocking peptides are peptides that bind specifically into the focus on antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged by the antibody.
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This tutorial outlines additional achievable optimization of mobile and immunoassay parameters to guarantee the very best outcomes are...
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This guideline outlines further possible optimization of mobile and immunoassay parameters to be sure the very best outcomes are...
Antibodies certain to the blocking peptide not bind to your epitope on the goal protein. By comparing the staining within the blocked antibody as opposed to the antibody by itself, you can see which staining is unique.
Target Information Cyclin E belongs on the remarkably conserved cyclin family members, whose associates are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which lead to your temporal coordination of every mitotic event. Cyclin E sorts a posh with and features being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates on the G1-S stage boundary and is degraded as cells development via S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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